❞ كتاب Bacteriological and epidemiological findings during examination of the uterine content of ewes with retention of fetal membranes ❝

Bacteriological and epidemiological findings during examination of the uterine content of ewes with retention of fetal membranes

Bacteriological and epidemiological findings during examination of the uterine content of ewes with retention of fetal membranes من كتب طب بيطرى

Bacteriological and epidemiological ®ndings during
examination of the uterine content of ewes
with retention of fetal membranes
A. Tzora
a
, L.S. Leontides
b
, G.S. Amiridis
b
,
G. Manos
a
, G.C. Fthenakis
b,*
a
Technological Educational Institution of Epirus, Arta, Greece
b
Department of Obstetrics and Reproduction, Veterinary Faculty, University of Thessaly,
P.O. Box 199, 43100 Karditsa, Greece
Received 18 January 2001; accepted 15 June 2001
Abstract
We included 92 pairs of ewes with or without retention of fetal membranes in a cohort study of 25
̄ocks in Southern Greece. We obtained two uterine content samples under aseptic conditions, by
introducing a swab into the uterus of these ewes, on the 2nd±4th and the 5th±9th day after lambing.
We used conventional bacteriological techniques to isolate and identify bacteria and to carry out
antimicrobial agents susceptibility testing. The prevalence of bacterial intrauterine contamination
among ewes with retention was 24% on the ®rst and 46% on the second sampling 6
P
<
0
:
0001) and
that among ewes without retention was 8 and 2% 6
P
>
0
:
05), respectively. Clinical signs accom-
panying the retention of fetal membranes were more frequently observed among ewes with
intrauterine contamination than among those without 6
P
ˆ
0
:
0007). The odds of an ewe having
an intrauterine contamination increased multiplicatively by 1.06 when the median duration of
retention in the ̄ock increased by 6 h. The principal bacteria isolated from the ewes with retention
were
Arcanobacterium pyogenes
and
Escherichia coli
; 21% of 73 isolates tested were found resistant
to at least one antimicrobial agent.
#
2002 Elsevier Science Inc. All rights reserved.
Keywords:
Sheep reproduction; Fetal membranes; Infection; Metritis;
Arcanobacterium pyogenes
1.Introduction
Postpartum intrauterine contamination has been studied extensively incows. Thegenital
tractisparticularlysusceptibletoinfectionsimmediatelyafter calving,butitisusuallyable
to overcome the nonspeci®c bacterial contamination [1]. In an early study,

93% of dairy
Theriogenology 57 62002) 1809±1817
*
Corresponding author. Tel.:
‡
30-932-641-379; fax:
‡
30-441-66041.
E-mail address:
gcf@vet.uth.gr 6G.C. Fthenakis).
0093-691X/02/$ ± see front matter
#
2002 Elsevier Science Inc. All rights reserved.
PII: S
0093-691X602)00684-2
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